Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary cells via multiple signaling pathways.

ABSTRACT:

Background

Chinese hamster ovary (CHO) cells are the predominant cell line used for biotherapeutic production. To reduce the cost of therapeutic recombinant proteins produced in CHO cells, efforts have been made over decades to improve overall yield through media and process optimization, as well as genetic engineering aimed at enhancing cell proliferation or productivity. Within an intricate cellular framework, such as the CHO cell system, it is indisputable that numerous genes with seemingly disparate functions may be involved in the process of expressing recombinant proteins.

Results

Thrombomodulin (TM), encoded by the Thbd gene, is primarily known for its roles in coagulation, innate immunity, inflammation, and tumor cell proliferation. Our research was the first to reve

SUBMITTER: Ma X 

PROVIDER: S-EPMC12606806 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature