Functional characterization of PcMYB25, a candidate regulator of Patchouli alcohol biosynthesis in Pogostemon cablin, via exogenous hormone-elicited transcriptome analysis.

ABSTRACT:

Background

Plant hormones often cause changes in secondary metabolites. Patchouli alcohol (PA) is a bioactive compound of the medicinal plant Pogostemon cablin. Similar to other secondary metabolites, its synthesis is also regulated by transcription factors. While the PA biosynthetic pathway has been characterized, its regulatory mechanisms remain incompletely understood.

Results

Transcriptome analysis revealed that exogenous hormone application significantly altered the transcriptional landscape of Pogostemon cablin, particularly affecting genes involved in terpenoid metabolism. Through weighted gene co-expression network analysis (WGCNA), we identified the R2R3-MYB transcription factor gene PcMYB25 as a key candidate regulator. Transient overexpression analysis showed that

SUBMITTER: Feng L 

PROVIDER: S-EPMC12606904 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature