LncRNA MEG8 promotes deep vein thrombosis by sponging miR-296-5p to regulate human umbilical vein endothelial cells.

ABSTRACT:

Aim

This study explored the diagnostic value and molecular mechanism of lncRNA MEG8 in deep vein thrombosis (DVT).

Methods

This study included 120 patients with DVT and 100 healthy individuals as research subjects. Expression of lncRNA MEG8 and miR-296-5p in subjects' serum were detected by RT-qPCR. Diagnostic ability of MEG8 for DVT occurrence analyzed by ROC curve. Logistic analysis was used to identify risk factors for DVT. Associations between MEG8 and other parameters were explored by Pearson correlation analysis. Migration, viability and apoptosis of transfected HUVECs were detected by Transwell method, CCK-8 assay and flow cytometry, respectively. In addition, inflammatory cytokines were detected using ELISA kits. The luciferase reporter assay established the interact

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12606911 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature