Shared M0 macrophage infiltration-related gene signatures in osteoarthritis and osteomyelitis by integrating bulk and single-cell RNA transcriptome.

ABSTRACT:

Background

Osteoarthritis (OA) and osteomyelitis (OM) are prevalent orthopedic diseases, characterized by complex immune and inflammatory responses that impact bone regeneration. Macrophages play a crucial role in the immune regulation of osteoblast function, with M0 macrophages polarizing toward M1 and M2 phenotypes, influencing pro-inflammatory or anti-inflammatory responses. Despite evidence linking M0 macrophages with inflammation in OA and OM, the underlying pathogenic and immune regulatory mechanisms remain unclear.

Methods

To examine the immune microenvironment in OA and OM, we analyzed 6 OA datasets and 3 OM datasets from the GEO database. Shared M0 macrophages-related differentially Expressed Genes (M0R-DEGs) in OA and OM were identified by differential expression a

SUBMITTER: Wei M 

PROVIDER: S-EPMC12607019 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature