Integrated bulk and single-cell transcriptomics identifies shared and specific immune signatures in Takayasu Arteritis.

ABSTRACT:

Background

Takayasu arteritis (TAK) is a rare, chronic large-vessel vasculitis. Although CD4⁺ and CD8⁺ T cells are acknowledged drivers of vascular injury in TAK, the gene networks that confer their pathogenicity remain incompletely mapped.

Objectives

This study aimed to integrate bulk RNA-sequencing of peripheral-blood T-cell subsets with single-cell RNA-sequencing of aortic tissue to find mechanistic biomarkers and therapeutic targets for TAK.

Methods

We performed bulk RNA-sequencing on peripheral-blood CD4⁺ and CD8⁺ T cells from eight treatment-naïve TAK patients and three age matched healthy controls. In parallel, single-cell RNA-sequencing was applied to aortic tissue from three additional TAK patients and three atherosclerotic controls. DEGs were defined at fals

SUBMITTER: Xu K 

PROVIDER: S-EPMC12607115 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature