Study on the mechanism of hUCMSCs-derived 3D exosome combined with HAMA microneedles in the treatment of deep second-degree burns.

ABSTRACT: Burn wound healing remains challenging due to uncontrolled inflammation and poor angiogenesis. We developed hyaluronic acid methacryloyl microneedles (EXO@HAMA) loaded with 3D-cultured mesenchymal stem cell (MSC) exosomes to overcome limitations of growth factor therapies and sudden release of exosomes. 3D-exosomes potently enhanced fibroblast proliferation and endothelial tube formation. In rat deep second-degree burns, EXO@HAMA reduced inflammation and accelerated healing through CTSB/TGF-β and Wnt/β-catenin axis modulation, significantly improving collagen deposition and angiogenesis. Transcriptomics revealed activation of lipid metabolism and extracellular matrix (ECM) remodeling. This work demonstrates 3D-exosome/HAMA synergy in resolving inflammation-angiogenesis imbalance, offering

SUBMITTER: Elakkawi MMJ 

PROVIDER: S-EPMC12607139 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature