MiR-27a-5p deficiency in plasma exosomes derived from obese individuals exacerbates ventilator-induced lung injury in vitro and in vivo.

ABSTRACT:

Background

Ventilator-induced lung injury (VILI) frequently occurs in obese individuals and significantly negates outcomes. Understanding mechanisms behind susceptibility of obese patients to VILI is, therefore, important. This study delved into the molecular biology mechanisms of VILI and investigated the effects of plasma exosomes from obese patients on VILI in mice, as well as its potential molecular mechanisms.

Methods

MicroRNAs (miRNAs) in plasma-derived exosomes from 6 obese patients and 6 healthy volunteers were analysed to map different miRNA profiles. A lung cell line (MLE-12) was cultured with or without exosomes from obese patients and then challenged with cyclic stretching for 4 h. Western blotting, immunofluorescence, electron microscopy and other techniques wer

SUBMITTER: Wang B 

PROVIDER: S-EPMC12607151 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature