Natural variation in tetrapyrrole biosynthetic enzymes and their regulation modifies the maize chlorophyll mutant Oy1-N1989.

ABSTRACT: Tetrapyrroles are macrocyclic compounds present in and required for all life on Earth. Mutants with defects in tetrapyrrole pathway enzymes can be used to uncover natural variation in this pathway and study pathway regulation. We report the effects of the Oil yellow1 mutation, Oy1-N1989, a semi-dominant allele of subunit I in the Mg-chelatase enzyme with reduced chlorophyll biosynthesis in maize (Zea mays), on global gene expression and chlorophyll content. In Oy1-N1989/+ mutants, coordinate feedback regulation of the tetrapyrrole pathway was observed as transcriptional feedback regulation of genes encoding steps in the tetrapyrrole pathway. Natural variation in the wild-type allele at oy1 modulated the severity of the impact of Oy1-N1989/+ on gene expression. Previously identified cis-act

SUBMITTER: Kaur A 

PROVIDER: S-EPMC12607265 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature