Characterization of a Novel <i>Galactia lindenii</i> Lectin and Its Effects on Lepidopteran Midgut Cells.

ABSTRACT: Lectins are carbohydrate-binding proteins involved in plant defense and have been widely explored for their insecticidal properties. While most lectins studied to date target pests from the orders Homoptera and Hemiptera, few are effective against Lepidoptera. This group includes highly destructive agricultural pests such as Spodoptera frugiperda. In this study, we report the purification and characterization of a novel Glc/Man-specific lectin, GLL-I, from the seeds of Galactia lindenii, an endemic Colombian legume. GLL-I was purified using ion exchange and affinity chromatography, and SDS-PAGE, isoelectric focusing, and mass spectrometry confirmed its identity. Structural analysis revealed a canonical legume lectin fold with high sequence similarity to ConA lectin. Functiona

SUBMITTER: Casas-Corredor Z 

PROVIDER: S-EPMC12607393 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature