Integrating genomic and Tn-Seq data to identify common <i>in vivo</i> fitness mechanisms across multiple bacterial species.

ABSTRACT: Sepsis, a life-threatening organ dysfunction, is due to an unregulated immune response to infection. Bacteremia is a leading cause of sepsis, and members of the Enterobacterales cause nearly half of bacteremia cases annually. Although previous Tn-Seq studies identified novel bacteremia-fitness genes, evidence for common pathways across species is lacking. To identify common fitness pathways in five bacteremia-causing Enterobacterales species, we utilized our pan-genome pipeline to integrate Tn-Seq fitness data with multiple available functional data types. Core genes from species pan-genomes were used to construct a multi-species core pan-genome, producing 2,850 core gene clusters found in four of five species. Integration of Tn-Seq fitness data identified 373 protein clusters conserved in

SUBMITTER: Fouts DE 

PROVIDER: S-EPMC12607656 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature