Short tandem gene duplications as potential agents of genetic memory.

ABSTRACT: Tandem gene duplications are spontaneous mutations that occur frequently but whose significance is often overlooked because they are genetically unstable and can be difficult to detect by sequencing. Here, we studied the spontaneous loss, amplification, and phenotypic effects of engineered duplications giving rise to antibiotic resistance in Pseudomonas aeruginosa. The duplications, ranging from 2.6 to 497 kb in length, were constructed using phage lambda red recombination of PCR products and carry resistance markers at their novel junctions. The genetic behaviors of the duplications depended on segment length: long duplications were readily lost during growth without selection and showed limited amplification potential, whereas short duplications were lost slowly and could a

SUBMITTER: Lee S 

PROVIDER: S-EPMC12607827 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature