Effect of antibiotics on Kupffer cell immunometabolism relative to intracellular killing of <i>S. aureus</i> using NAD(P)H fluorescence lifetime imaging.

ABSTRACT: Staphylococcus aureus bacteremia (SAB) remains a significant clinical burden with high mortality rates. Kupffer cells (KCs) are integral to clearing Staphylococcus aureus (SA) from the bloodstream yet also serve as a pathogenic reservoir, driving persistence and treatment failure. KCs utilize their metabolic plasticity to coordinate successful antimicrobial responses, and this process may be therapeutically targeted. We investigated the immunometabolic effects of antistaphylococcal antibiotics (vancomycin, ceftobiprole, daptomycin, and tedizolid) on KCs relative to clearance of intracellular SA through gene expression analysis and optical metabolic imaging using fluorescence lifetime imaging microscopy. We observed differential effects of antibiotics on KC polarization and me

SUBMITTER: Beadell B 

PROVIDER: S-EPMC12607893 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature