Inhibition of HIV-1 replication by primer RNA packaging inhibitors.

ABSTRACT: An essential step in the life cycle of human immunodeficiency virus type 1 (HIV-1) is the packaging of tRNA₃^Lys during viral assembly. This step, which requires the assembly of a ternary complex consisting of GagPol, mitochondrial lysyl-tRNA synthetase, and tRNA₃^Lys, is essential for the production of infectious viruses. Indeed, it allows the initiation of reverse transcription in the particles. In this work, we used an in vitro assay to isolate molecules from a chemical library that disrupt the interaction between the integrase domain of GagPol and mitochondrial lysyl-tRNA synthetase. The three selected molecules also inhibit HIV-1 replication in an ex vivo assay. The number of HIV-1 particles produced in the presence of the drugs

SUBMITTER: Mirande M 

PROVIDER: S-EPMC12607897 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature