Molecular insights into nucleocapsid assembly and transport in Marburg and Ebola viruses.

ABSTRACT: Live-cell imaging enables visualization of the spatiotemporal dynamics of signals in cells. Intracytoplasmic movement of nucleocapsids is crucial during the life cycle of enveloped viruses; however, the molecular mechanisms governing their assembly and transport are not fully understood. Using a Marburg virus (MARV) live-cell imaging system, we identified three nucleocapsid proteins-nucleoprotein (NP), VP35, and VP24-that are necessary and sufficient to form transport-competent nucleocapsid-like structures (NCLSs). These findings are consistent with observations in Ebola virus (EBOV). Interestingly, despite incompatibility among these proteins, VP30 interacts with nucleocapsid proteins from both MARV and EBOV, supporting viral transcription and replication in heterologous systems. Furtherm

SUBMITTER: Takamatsu Y 

PROVIDER: S-EPMC12607913 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature