Bioinformatics Analysis of the Tomato <i>SlPR5</i> Gene Family and the Thaumatin-like Protein SlPR5-3 Positively Regulates Tomato Resistance to <i>Pst</i> DC3000.

ABSTRACT: Studies on how SlPR5 genes are involved in Pst DC3000 disease resistance response are lacking. Here, 27 members of the tomato SlPR5 gene family were identified and analyzed. Analysis of conserved structural domains and promoter structure revealed that SlPR5 family members are structurally conserved and contain a variety of antidisease and antistress response elements. We screened out SlPR5-3, which was significantly upregulated, through an analysis of the expression pattern of Pst DC3000 in tomato after inoculation. We generated SlPR5-3 mutants via CRISPR/Cas9 gene editing and SlPR5-3-overexpressing tomato plants to elucidate the function of this gene. The results showed that the SlPR5-3 overexpression lines had reduced lesions

SUBMITTER: Pang X 

PROVIDER: S-EPMC12608184 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature