Exploring the Biochemical Mechanism Beyond the Cytotoxic Activity of Sesquiterpene Lactones from Sicilian Accession of <i>Laserpitium siler</i> Subsp. <i>siculum</i> (Spreng.) Thell.

ABSTRACT: Laserpitium siler subsp. siculum (Apiaceae) is a Mediterranean plant with a long history of traditional medicinal use. In this study, the chemical composition and anticancer potential of three novel (and one new to the genus) sesquiterpene lactones (SLs) isolated from its roots were investigated. The structural characterization, carried out through NMR and HPLC-MS analyses, identified unique guaianolide-type lactones. The biological activity of these compounds was evaluated in vitro using MDA-MB-231 cells, a triple-negative breast cancer (TNBC) cell line. Cell viability assays demonstrated that all SLs tested reduced TNBC cell viability in a dose- and time-dependent manner, with SL-1 exhibiting the highest cytotoxicity. Light microscopy analyses and acridine orange/ethidium b

SUBMITTER: Vaglica A 

PROVIDER: S-EPMC12608240 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature