Temporal Analysis of Embryonic Epidermal Morphogenesis in <i>Caenorhabditis elegans</i>.

ABSTRACT: The development of epidermis plays a central role in driving the morphogenesis of the Caenorhabditis elegans embryo. However, current research on epidermal morphogenesis focuses disproportionately on overt phenotypic abnormalities, potentially overlooking the crucial role of developmental timing. In this study, we developed a modular two-step deep learning-based image analysis pipeline. First, we used ResU-Net to extract completely developed embryos and suppress noise; second, ResNet was used to predict the corresponding embryonic stage. The predicted probabilities and their corresponding embryonic time points were subsequently utilized to construct a developmental timeline. Combining this pipeline with differential interference contrast time-lapse microscopy, we dynamically tracked

SUBMITTER: Li F 

PROVIDER: S-EPMC12608371 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature