Exosomal microRNA Panels for Detecting Early-Stage Non-Small Cell Lung Cancer.

ABSTRACT: Background: Early diagnosis of lung cancer requires lung nodule biopsies, which can lead to severe complications. This study aimed to identify optimized panels of exosomal microRNAs (miRNAs) for non-invasive diagnosis of early-stage non-small cell lung cancer (NSCLC). Materials and Methods: This study comprised four phases: discovery, validation, optimization, and confirmation. In the discovery phase, next-generation sequencing profiled 2656 exosomal miRNAs in serum samples (n = 76) from patients with benign lung nodules and stage-specific NSCLC. The validation phase used qPCR to analyze selected miRNAs in serum samples (n = 75). The optimization phase employed a self-devised diagnostic platform, the "up-down ratio (UDR)," to identify miRNA panels. The confirmat

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC12608640 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature