Urea Detection in Phosphate Buffer and Artificial Urine: A Simplified Kinetic Model of a pH-Sensitive EISCAP Urea Biosensor.

ABSTRACT: A simplified kinetic model for the quantitative analysis of a potentiometric, pH-based urea biosensor is presented. The device was an electrolyte-insulator-semiconductor capacitor (EISCAP) with a pH-sensitive Ta₂O₅ gate functionalized by a polyallylamine hydrochloride (PAH)/urease bilayer. Within the steady-state approximation, the kinetic equations yielded an implicit algebraic relation linking the bulk urea concentration to the local pH at the sensor surface. Numerical solution of this equation, combined with a fitting routine, provides the apparent Michaelis-Menten constant (KM) and the normalized maximum reaction rate (k¯V). Validation against the literature data confirmed the reliability of the approach. Experimental results were then analyzed in both phosphate b

SUBMITTER: Simonyan K 

PROVIDER: S-EPMC12608829 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature