Molecular Identification and Biogenic Amine Production Capacity of <i>Enterococcus faecalis</i> Strains Isolated from Raw Milk.

ABSTRACT: In this study, Enterococcus faecalis strains isolated from raw cow's milk were examined for genetic diversity, ability to produce biogenic amines (including histamine, tyramine, putrescine, cadaverine, 2-phenylethylamine) and the presence of corresponding amino acid decarboxylase genes. Identification of 29 strains obtained from Polish farms was carried out by polymerase chain reaction (PCR) and matrix-assisted laser desorption and ionization time of flight (MALDI-TOF MS) methods, and their genetic relationships were assessed by the Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) technique. Amine production capacity was assessed in vitro on synthetic medium, while the presence of decarboxylase genes (hdcA, tyrS, tyrDC, Odc

SUBMITTER: Wisniewski P 

PROVIDER: S-EPMC12608866 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature