In Silico Characterisation and Determination of Gene Expression Levels of the <i>CPK</i> Family Under Saline Stress Conditions in <i>Chenopodium quinoa</i> Willd.

ABSTRACT: Quinoa (Chenopodium quinoa Willd.) is a highly nutritious crop known for its tolerance to salt stress; however, the molecular mechanisms underlying this trait remain poorly understood. This study aims to perform the in silico characterisation of calcium-dependent protein kinase (CPK) gene family sequences and to evaluate their expression profiles under salt stress conditions. Using bioinformatics tools, CPK family gene sequences were identified and in silico-characterised, including conserved domains, cis-regulatory motifs, and physicochemical properties. Experimentally, two contrasting accessions were compared: a salt-tolerant one (UNSA_VP033) and a salt-sensitive one (UNSA_VP021). Salt tolerance indices were determined during germination, gene expression levels were quantif

SUBMITTER: Lima-Huanca L 

PROVIDER: S-EPMC12609707 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature