Altered Development of Gut Microbiota and Gastrointestinal Inflammation in Children with Post-Operative Hirschsprung's Disease.

ABSTRACT: Gastrointestinal symptoms often persist in children with Hirschsprung's disease (HD) even after "corrective" pull-through surgery. Alteration of the gut microbiota ("dysbiosis") has emerged as a potential contributing factor. Animal studies show gut ecosystem changes that are both intrinsic to HD and caused by bowel resection itself, but human studies comparing the intestinal microbiota of children with HD and healthy children are limited. We collected food frequency dietary surveys, clinical and symptom data, and stool samples from 15 post-operative children with HD and 15 healthy controls (HCs). We performed 16S rRNA gene sequencing from the stool samples and quantified faecal calprotectin as a measure of gastrointestinal inflammation. Despite no global changes in the microbiota between

SUBMITTER: Murphy CE 

PROVIDER: S-EPMC12609776 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature