Protein Phosphatase OsPP2C55 Negatively Regulates Abscisic Acid Biosynthesis and Saline-Alkaline Tolerance in Rice.

ABSTRACT: In rice (Oryza sativa L.), the short-chain dehydrogenase protein OsABA2 plays a crucial role in regulating abscisic acid (ABA) biosynthesis. However, little is known about the other proteins that interact with OsABA2 to regulate ABA biosynthesis. Using yeast two-hybrid screening, we identified a novel OsABA2 interacting protein OsPP2C55, which contains a serine/threonine phosphatase (family 2C) catalytic domain. The yeast two-hybrid (Y2H) assay and firefly luciferase complementary imaging (LCI) assay confirmed these interactions. Subsequent studies revealed that saline-alkaline stress significantly downregulated OsPP2C55 gene expression. Meanwhile, we constructed ospp2c55 CRISPR gene knockout (ospp2c55-KO) plants using Agrobacterium genetic transformation. Compa

SUBMITTER: Zhang G 

PROVIDER: S-EPMC12610132 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature