Structural Engineering of Tyrosine-Based Neuroprotective Peptides: A New Strategy for Efficient Blood-Brain Barrier Penetration.

ABSTRACT: The relationship between the structure of walnut-derived peptides and their activity of transport efficiency across the blood-brain barrier (BBB) remains unclear. In this study, a series of walnut-derived peptides were synthesized by substituting leucine (L) with tyrosine (Y), lysine (K), or arginine (R). Three outstanding peptides-EVSGPGYSPN, TWLPYPR, and YVPFPYP-were selected based on their antioxidant capacity and BBB transport efficiency, with EVSGPGYSPN exhibiting the highest activity. Reversed-phase high-performance liquid chromatography (RP-HPLC) and Transwell assay results demonstrated that EVSGPGYSPN can remain stable during gastrointestinal digestion and penetrate the BBB. Pharmacokinetic results revealed that the cumulative concentration of EVSGPGYSPN in the brain reached 1.25 ±

SUBMITTER: Li Z 

PROVIDER: S-EPMC12610161 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature