Influence of Isolation Techniques on the Quality of Plasma Samples: Implications for Cancer Biobanking.

ABSTRACT: Biobanks are essential for precision oncology, providing high-quality materials for biomedical research. Liquid biopsy has become a key tool for non-invasive detection of tumor-derived biomarkers, including circulating tumor DNA, proteins, and extracellular vesicles. However, the reliability of these assays critically depends on standardized preanalytical procedures. In this study, we evaluated the impact of two plasma isolation methods-direct centrifugation (DC) and density gradient centrifugation (DGC)-on the overall quality of breast cancer samples collected at the Bruno Boerci Biobank (Maugeri, Italy). Plasma obtained with the two methods was analyzed by spectrometry for hemolysis and lipemia, biochemical analysis for protein and lipoprotein composition, flow cytometry for cellular deb

SUBMITTER: Piccotti F 

PROVIDER: S-EPMC12610171 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature