The Barley Glycosyltransferase Gene <i>KOB1</i> Implicated in β-Glucan Biosynthesis by a Genome-Wide Association Study.

ABSTRACT: β-glucan, a crucial trait in barley breeding programs, serves as a quality determinant of products intended for both human consumption and animal feed. Although genes involved in β-glucan synthesis have been reported, the genetic mechanisms regulating its accumulation in barley grain remain underexplored. In this study, we functionally characterized KOB1, a candidate gene identified from a genome-wide association study (GWAS) on barley seed β-glucan content, which encodes a glycosyltransferase. Haplotype analysis showed that haplotype E was associated with significantly elevated grain β-glucan levels compared to other haplotypes. Furthermore, overexpression of KOB1 in rice led to a significant increase in grain β-glucan content relative to the wild-type Zhonghua 11, confirmin

SUBMITTER: Wan G 

PROVIDER: S-EPMC12610209 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature