The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.

ABSTRACT: Starch is an important carbohydrate in lotus (Nelumbo nucifera Gaertn.) roots, which are edible rhizomes, and starch biosynthesis and metabolism strongly influence lotus root yield and quality. Plant microRNAs (miRNAs) have a significant effect on crop yield, quality, and starch biosynthesis, but the molecular mechanism by which miRNAs regulate starch biosynthesis is unknown. In this study, miR395b expression levels showed significant differences in lotus germplasms with different starch contents. Overexpressing MIR395b reduced amylopectin levels and medium- and long-branched chain percentages in lotus roots, decreasing total starch accumulation. Inhibiting miR395b function (using short tandem target mimics) or overexpressing ABA Insensitive 5 (NnABI5) increased amylopectin content and med

SUBMITTER: Li F 

PROVIDER: S-EPMC12610401 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature