Optimization of Micropropagation and Metabolomic Analysis Under Different Light Qualities in <i>Mussaenda pubescens</i> Ait.f.

ABSTRACT: The current investigation utilized stem nodes from pre-established aseptic lines of Mussaenda pubescens as explants to optimize an efficient in vitro propagation protocol and investigated the effect of different light qualities (white, red, blue, and green) on metabolite accumulation in micropropagated plantlets. The findings demonstrated that the optimal medium for shoot proliferation was Murashige and Skoog basal medium supplemented with 6-Benzylaminopurine 2.0 mg·L^-1 and α-naphthaleneacetic acid 0.2 mg·L^-1, achieving a multiplication coefficient of 12.2 after 30 days. Rooting was more effective on Murashige and Skoog basal medium containing α-naphthaleneacetic acid 0.1 mg·L^-1 and activated charcoal 1 g·L^-1, resulting in a 100% rooting r

SUBMITTER: Sun L 

PROVIDER: S-EPMC12610484 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature