Influence of Nitrate and Light on Fucoxanthin Content and Key Gene Expression in the Marine Diatom <i>Thalassiosira rotula</i>.

ABSTRACT: Fucoxanthin is the predominant carotenoid in diatoms, playing a central role in light harvesting and photoprotection, and is increasingly valued for its potential in pharmaceutical, nutraceutical, and cosmetic applications. In this study, we investigated the influence of high nitrate supplementation, low-light exposure, and combined treatment, on fucoxanthin content and on the expression of key genes involved in its biosynthetic pathway in the marine diatom Thalassiosira rotula. Fucoxanthin content was quantified using HPLC-based and spectrophotometric methods. Control culture at the exponential growth phase showed a fucoxanthin content of 4.7 mg g^-1 DW, reaching 5.2 mg g^-1 DW under low-light conditions at the late exponential phase. Gene expression analysis re

SUBMITTER: Madeo ML 

PROVIDER: S-EPMC12610687 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature