Integrative Transcriptomic and Proteomic Analysis Reveals <i>CaMK4</i>-Mediated Regulation of Proliferation in Goat Skeletal Muscle Satellite Cells.

ABSTRACT: CaMK4, a calcium/calmodulin-dependent protein kinase, is an important mediator of cellular signal transduction, yet its role in the regulation of skeletal muscle satellite cells (MuSCs) in goats has remained unclear. In this study, CaMK4 overexpression and knockdown models were established, and integrated transcriptomic and proteomic analyses were performed to systematically elucidate its regulatory network. CaMK4 overexpression altered key pathways associated with cell proliferation and muscle development, including cAMP, PI3K-Akt, and actin cytoskeleton regulation, while proteomic data highlighted calcium signaling and JAK-STAT pathways. Conversely, CaMK4 knockdown enhanced MuSC proliferation by upregulating cell cycle-related genes and proteins. Integrated an

SUBMITTER: Cong H 

PROVIDER: S-EPMC12610772 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature