In Vitro Gastrointestinal Digestion of <i>Grifola frondosa</i> Polysaccharides and Their Enhancement of GABA Production via Gut Microbiota Modulation.

ABSTRACT: Background: The water-soluble Grifola frondosa polysaccharides (GFPs) are the primary bioactive component of the edible and medicinal fungus Grifola frondosa. However, the digestive behavior of GFPs in the human gastrointestinal (GI) tract and their subsequent interaction with gut microbiota (GM) to exert health effects remain unclear. Methods: In this study, GFPs were extracted based on a traditional hot water decoction. An in vitro simulated GI digestion model and a human fecal microbiota fermentation model were established to systematically investigate the digestive stability of GFPs, GM modulation, and metabolite changes. Results: Results showed that GFPs remained structurally stable during in vitro oral, gastric, and small intestinal digestion, allow

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12610785 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature