Enhanced Solubility and Electron Transfer of Osmium-Based Mediators via Quaternized Poly(4-Vinylpyridine) for Electrochemical Glucose Detection.

ABSTRACT: Hydrophilic polymer-osmium complexes enhance electron transfer between enzymes and electrodes in biosensors. In this study, hydrophobic poly(4-vinylpyridine) (PVP) was quaternized with 2-bromoethanol to synthesize water-soluble PVP(Q)-C₂H₄OH polymers (MW 60,000 and 160,000). The resulting PVP(Q)-C₂H₄OH-Os(dmo-bpy)₂Cl complexes were verified by UV-Vis, FT-IR, 1H NMR, SEM-EDS, and zeta potential analyses, confirming successful quaternization and osmium coordination with good dispersion stability. Electrochemical tests (cyclic voltammetry, multi-potential step, amperometry) demonstrated that electrodes with quaternized mediators showed greatly enhanced catalytic currents for glucose (0-20 mM), with sensitivities of 6.9791 (MW 60,000) and

SUBMITTER: Cho YY 

PROVIDER: S-EPMC12610834 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature