In silico structural analysis of Oryza sativa RAD51 reveals key interactions for nucleoprotein filament assembly and regulation.

ABSTRACT: Radiation sensitivity 51 (RAD51) is important for homologous recombination and DNA repair. The interaction between BRCA2 and RAD51 is crucial for the successful repair of DNA double strand breaks by homologous recombination. In the present study, through in silico analysis, we structurally characterized OsRAD51, a eukaryotic RAD51 ortholog from Oryza sativa Japonica A1 cultivar. Multiple sequence alignment showed the presence of conserved amino acids at the ATP- and DNA-binding sites. Several phosphorylation and ubiquitination sites were also predicted in OsRAD51 indicating its regulation by post-translational modifications. Structural modelling of OsRAD51 revealed two important regions at the protomer interface - one near the ATP-binding site (Walker A motif) and the other comprising of m

SUBMITTER: Azeem A 

PROVIDER: S-EPMC12611145 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature