Diploid assembly of the Mexican lime genome.

ABSTRACT: Many citrus species show high levels of heterozygosity due to their hybrid origin and clonal propagation. This heterozygosity can both hinder and aid efforts to study and improve these cultivars, making it increasingly clear that diploid assemblies have significant advantages over the previous generation of haploid assemblies. In this work, we assemble both subgenomes of Mexican lime (Citrus × aurantifolia), an interspecific hybrid between Citrus hystrix var. micrantha and Citrus medica. The resulting diploid assembly is nearly telomere-to-telomere, spanning 680 Mb. Using subgenome-specific repeats, we were able to phase the 18 chromosomes based on their parent of origin. The resulting hystrix and medica haplotypes show a number of large structural variations, consistent with their distant

SUBMITTER: Massaro I 

PROVIDER: S-EPMC12611252 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature