Transcription factor-mediated lineage switching reveals plasticity in primary committed progenitor cells.

ABSTRACT: The developmental plasticity of transplanted adult stem cells challenges the notion that tissue-restricted stem cells have stringently limited lineage potential and prompts a re-evaluation of the stability of lineage commitment. Transformed cell systems are inappropriate for such studies, since transformation potentially dysregulates the processes governing lineage commitment. We have therefore assessed the stability of normal lineage commitment in primary adult haematopoietic cells. For these studies we have used prospectively isolated primary bipotent progenitors, which normally display only neutrophil and monocyte differentiation in vitro. In response to ectopic transcription factor expression, these neutrophil/monocyte progenitors were reprogrammed to take on erythroid, eosinophil and

SUBMITTER: Heyworth C 

PROVIDER: S-EPMC126114 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature