Proteomic analysis of exosomes from <i>Brucella abortus</i>-infected macrophages reveals possible mechanisms of immune evasion and host modulation.

ABSTRACT:

Introduction

Brucella abortus is an intracellular pathogen that establishes chronic infections through immune evasion. Exosomes, a subtype of extracellular vesicles, mediate intercellular communication and can modulate host immune responses during infection. However, the proteomic composition and functional significance of exosomes from B. abortus-infected macrophages remain unclear.

Methods

Exosomes were isolated from RAW 264.7 macrophages infected or uninfected with B. abortus strain 2308, at 8 and 24 hours post-infection (hpi), using sequential centrifugation and immunoaffinity capture. Size and morphology were assessed by nanoparticle tracking analysis and transmission electron microscopy. Proteins were identified and quantified by label-free LC-MS/M

SUBMITTER: Alvarez F 

PROVIDER: S-EPMC12611657 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature