A new paradigm for outer membrane protein biogenesis in the Bacteroidota.

ABSTRACT: In Gram-negative bacteria, the outer membrane is the first line of defence against antimicrobial agents and immunological attacks¹. A key part of outer membrane biogenesis is the insertion of outer membrane proteins by the β-barrel-assembly machinery (BAM)^2-4. Here we report the cryo-electron microscopy structure of a BAM complex isolated from Flavobacterium johnsoniae, a member of the Bacteroidota, a phylum that includes key human commensals and major anaerobic pathogens. This BAM complex is extensively modified from the canonical Escherichia coli system and includes an extracellular canopy that overhangs the substrate folding site and a subunit that inserts into the BAM pore. The novel BamG and BamH subunits that are involved in forming the extracellular canopy are

SUBMITTER: Liu X 

PROVIDER: S-EPMC12611786 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature