Multiplex PCR-based rapid pathogen identification in acute cholecystitis using the FilmArray BCID2 panel.

ABSTRACT:

Purpose

Acute biliary infections are serious conditions in which delayed or inappropriate therapy can worsen clinical outcomes and promote antimicrobial resistance. This study evaluated the FilmArray® Blood Culture Identification 2 (BCID2) panel, a multiplex PCR system, for the rapid detection of pathogens in bile samples from patients with acute cholecystitis.

Methods

Bile samples were collected intraoperatively or by percutaneous aspiration from 77 consecutive patients with acute cholecystitis. Each specimen was divided for conventional culture and BCID2 panel testing. Culture results served as the reference standard to calculate positive and negative percent agreement (PPA and NPA), predictive values, and accuracy.

Results

Bacterial growth was observed in 34 cases

SUBMITTER: Nakamura G 

PROVIDER: S-EPMC12611922 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature