Alterations in renal Na,K-ATPase activity and protein expression in rat models of pressure and volume overload.

ABSTRACT:

Purpose

To explore an unexamined mechanism of cardiorenal pathophysiology by assessing renal Na, K-ATPase kinetics in rat models of pressure overload, volume overload, and their combination.

Methods

Two rat models with differing renin-angiotensin-aldosterone system activity were used: control Hannover Sprague Dawley (HAN) rats and transgenic TGR(mREN2)27 rats, the later modeling pressure overload. Each model included sham and aortocaval fistula (ACF)-operated groups to induce volume overload. The kinetic parameters of Na,K-ATPase were determined: maximal velocity of enzyme reaction (V_max), and the Michaelis constant (K_m), representing the ATP concentration at half-maximal velocity and reflecting the enzyme's affinity for ATP.

Results

Histological

SUBMITTER: Vrbjar N 

PROVIDER: S-EPMC12612110 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature