Subtractive proteomic analysis to identify highly antigenic proteins and reverse vaccinology based novel vaccine development against Haemophillius parainfluenzae.

ABSTRACT: Haemophillius parainfluenzae (H. parainfluenzae) is increasingly involved in invasive infections disrupting normal skin or mucosal barriers, specifically in immunocompromised individuals. No specific therapeutics or licensed vaccines are available for H. parainfluenzae infections. In the present study, the genome-scan vaccinomics analysis ranked three potential vaccine candidate proteins based on high immunogenic parameters, which were further used to prioritize lead B- and T-cell epitopes. The top-ranked highly immunogenic epitopes were conjugated using suitable linkers (KK, AAY, and GPGPG) and adjuvant (beta-defensin 3) sequences to engineer a potential chimeric vaccine construct. The immunological and physiochemical properties of the designed H. parainfluenzae vaccine exhibited high imm

SUBMITTER: Ahmad A 

PROVIDER: S-EPMC12612167 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature