Osteopontin promoted cardiac inflammation through increased interleukin-12 in acute myocarditis.

ABSTRACT: Osteopontin (OPN) is a multifunctional glycoprotein known to play critical roles in autoimmunity and tissue repair, yet its function in acute viral myocarditis remains poorly understood. To systematically investigate the pathogenesis of viral myocarditis, this study integrated clinical observations from patients with myocarditis and four genetically distinct mouse strains following intraperitoneal inoculation with coxsackievirus B3 (CVB3). Cardiac transcriptomic profiling via RNA-seq uncovered global changes in gene expression, while quantitative RT-PCR and ELISA assays were employed to measure OPN expression levels in cardiac tissues and plasma. A macrophage-specific OPN knockout (OPN^-/-) model was generated by crossing OPN flox/flox mice with Lyz2-Cre transgenic mice. In patie

SUBMITTER: Nie X 

PROVIDER: S-EPMC12612485 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature