Uclacyanin MtUC1 Is Involved in the Regulation of Nodule Senescence in Medicago truncatula.

ABSTRACT: Phytocyanins (PCs) are ancient plant-specific blue copper proteins that play an important role in plant growth and development, and stress tolerance. In this study, the role of MtUC1, a member of the uclacyanin subfamily of the PC family, was analysed in the nodule symbiosis of Medicago truncatula. MtUC1 was mainly expressed in the nodule interzone and strongly induced in the later nodule developmental stage. RNA interference (RNAi) and mutation of MtUC1 led to reduced root nodule formation and degeneration of bacteroids within nodules. Cysteine protease activity in the MtUC1-RNAi inoculated roots and uc1 mutant nodules was significantly increased, the leghaemoglobin content and the expression of nitrogen-fixing enzyme genes in the uc1 mutant nodules were significantly reduced, and the nod

SUBMITTER: Wang L 

PROVIDER: S-EPMC12612560 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature