A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample.

ABSTRACT:

Background

Presently available flow cytometric methods of bromodeoxyuridine (BrdUrd) labelling do not provide information on the cell cycle time (TC) and the growth fraction (GF). In this paper, we describe a novel and simple method to estimate TC and GF from flow cytometric analysis of a single tumour sample after BrdUrd labelling.

Methods

The proposed method is based on two assumptions: (1) the number of labelled cells traversing the cell cycle per unit time is constant and (2) the total number of labelled cells is constant throughout the cycle, provided that cells produced after division are excluded. The total numbers of labelled divided G1 cells, labelled divided S cells, labelled undivided S cells, and labelled undivided G2 cells were obtained for DNA histograms of Brd

SUBMITTER: Eidukevicius R 

PROVIDER: S-EPMC1261259 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature