Characterization of the global profile of genes expressed in cervical epithelium by Serial Analysis of Gene Expression (SAGE).

ABSTRACT:

Background

Serial Analysis of Gene Expression (SAGE) is a new technique that allows a detailed and profound quantitative and qualitative knowledge of gene expression profile, without previous knowledge of sequence of analyzed genes. We carried out a modification of SAGE methodology (microSAGE), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions. Cervical epithelium is constituted mainly by cervical keratinocytes which are the targets of human papilloma virus (HPV), where persistent HPV infection of cervical epithelium is associated with an increase risk for developing cervical carcinomas (CC).

Results

We report here a transcriptome analysis of cervical tissue by SAGE, derive

SUBMITTER: Perez-Plasencia C 

PROVIDER: S-EPMC1261262 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature