Construction and validation of a Bovine Innate Immune Microarray.

ABSTRACT:

Background

Microarray transcript profiling has the potential to illuminate the molecular processes that are involved in the responses of cattle to disease challenges. This knowledge may allow the development of strategies that exploit these genes to enhance resistance to disease in an individual or animal population.

Results

The Bovine Innate Immune Microarray developed in this study consists of 1480 characterised genes identified by literature searches, 31 positive and negative control elements and 5376 cDNAs derived from subtracted and normalised libraries. The cDNA libraries were produced from 'challenged' bovine epithelial and leukocyte cells. The microarray was found to have a limit of detection of 1 pg/microg of total RNA and a mean slide-to-slide correlation co-effici

SUBMITTER: Donaldson L 

PROVIDER: S-EPMC1261263 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature