Quantitative SERS-Based Sandwich-Hybridization Assay for Nucleic Acid Detection.

ABSTRACT: Surface-enhanced Raman spectroscopy (SERS) has great potential for quantifying biological molecules, particularly DNA. In this study, we developed a SERS-based sandwich-hybridization assay for detecting DNA corresponding to viral (SARS-CoV-2) RNA using commercially available components and standard bioconjugation procedures; hence, no sophisticated synthesis steps were required. The assay employs TAMRA-labeled DNA conjugated to gold nanoparticles as nanoprobes and biotin-labeled surface DNA strands. Upon hybridization with the target analyte, the complete sandwich structure forms and is captured on a neutravidin-coated glass via the avidin-biotin interaction. The observed Raman signals originate from the TAMRA reporter dye, leveraging signal enhancement from both resonance and plasmonic ef

SUBMITTER: Shahsavar K 

PROVIDER: S-EPMC12612968 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature