<i>Pseudomonas aeruginosa</i> affects <i>Acinetobacter baumannii</i>'s growth, gene expression and antibiotic resistance in <i>in vitro</i> co-culture system.

ABSTRACT: Pseudomonas aeruginosa and Acinetobacter baumannii are opportunistic pathogens co-isolated frequently in the same infection sites. In this study, we aimed to elucidate the interaction between P. aeruginosa and A. baumannii in in vitro co-culture system. Growth analysis showed that P. aeruginosa PA01 unilaterally exerts a negative effect on the growth of A. baumannii SK005. A. baumannii alone exhibited an altered transcriptome pattern, especially in the genes for metabolic pathways. Strong upregulation of genes in iron acquisition systems (acinetobactin, baumannoferrin, 2,3-dihydroxybenzoic acid synthesis cluster, and Feo iron transport system) and downregulation of iron-storage genes (bacterioferritin) were the most prominent changes.

SUBMITTER: Phoo MTP 

PROVIDER: S-EPMC12613057 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature