Real-Time Binding Kinetics of Membrane Protein-Protein Interactions in a Membraneless Setting.

ABSTRACT: A ubiquitous problem in protein analytics and medical biotechnology is assessing the interaction of a membrane protein receptor with its cognate protein ligand. This task generally requires transferring the receptor from native membranes or other expression host systems into supported lipid bilayers, liposomes, or nanodiscs. Such a reintegration process necessitates multiple steps for protein solubilization, renaturing, and functional reconstitution. Here, we opportunistically show that biolayer interferometry (BLI) can be directly utilized to evaluate the pre-equilibrium binding kinetics of a membrane protein receptor with its protein ligand in a label-free and membraneless setting. We present real-time measurements probing the association and dissociation phases of these transient comple

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12613146 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature