Development of a Novel Homogeneous Liposome-Based One-Step Assay for SARS-CoV-2 Antibody Detection in Human Serum Based on Fluorescent Liposomes and Complement Activity.

ABSTRACT: Monitoring antibodies in patient serum enables the diagnosis of infectious and chronic diseases, the assessment of an individual's immune status, and possible protection against infection and could thus be a ubiquitous tool for pandemic preparedness and personalized medicine. Advancing from the traditional enzyme-linked immunosorbent assay (ELISA), a homogeneous assay format was developed that simplifies assay procedures and enables a wash-free one-step performance. SARS-CoV-2 was chosen as the model virus, and its Spike protein-derived receptor binding domain (RBD) was covalently coupled to fluorescent liposomes. The binding of patient antibodies triggered the complement system, led to liposome lysis, and allowed quantitative fluorescent detection. The liposome assay was optimized with re

SUBMITTER: Reiner C 

PROVIDER: S-EPMC12613147 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature