Trapped Ion Mobility Improves Annotation Accuracy in LC-HRMS Screening Applications for Exposomics.

ABSTRACT: Ion mobility techniques coupled to mass spectrometry, such as trapped ion mobility (TIMS), are promoted to separate analytes from coeluting matrix interferences and to resolve isomers based on their corresponding CCS values. Complementary to the retention time (RT) dimension revealed from liquid chromatography, the collision cross section (CCS) serves as a robust and matrix-independent parameter. We evaluated the advantages of TIMS in the screening of human samples, such as urine, serum, breastmilk, and matrices relevant for exposure analysis, such as dust and wastewater. We conducted a screening library for 769 environmental contaminants, which resulted in a total of 948 CCS values (594 positive and 354 negative ionization modes). We screened for the potential co-occurrence of interfering

SUBMITTER: Huber C 

PROVIDER: S-EPMC12613149 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature